Optimization of a protein extraction technique for fungal proteomics
نویسندگان
چکیده
منابع مشابه
Optimization of Urea Based Protein Extraction from Formalin-Fixed Paraffin-Embedded Tissue for Shotgun Proteomics
Urea based protein extraction of formalin-fixed paraffin-embedded (FFPE) tissue provides the most efficient workflow for proteomics due to its compatibility with liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). This study optimizes the use of urea for proteomic analysis of clinical FFPE tissue. A series of protein extraction conditions manipulating temperat...
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چکیده ندارد.
15 صفحه اولTools for fungal proteomics: multifunctional neurospora vectors for gene replacement, protein expression and protein purification.
The completion of genome-sequencing projects for a number of fungi set the stage for detailed investigations of proteins. We report the generation of versatile expression vectors for detection and isolation of proteins and protein complexes in the filamentous fungus Neurospora crassa. The vectors, which can be adapted for other fungi, contain C- or N-terminal FLAG, HA, Myc, GFP, or HAT-FLAG epi...
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ژورنال
عنوان ژورنال: Indian Journal of Microbiology
سال: 2010
ISSN: 0046-8991,0973-7715
DOI: 10.1007/s12088-010-0072-3